Sample processing (extraction, purification, concentration, etc.)

Is the addition of an internal standard required?
Analytical replicates per sample (the cost of analysis is based on the number of instrument injections)
Method validation requirements (additional fees applicable)
For more information, see How to validate analytical methods (Wood, 1999)

Do the samples contain any state/federal regulated substances (even trace amount of such substances should be disclosed, and consulted with Clemson EHS)?